The air mixes with the recirculating air stream and passes through a hepa filter downward toward the work surface creating a contamination free zone.
Uses and principle of operation of biosafety cabinet.
With proper techniques activities within the work area can be performed safely minimizing the chance of cross contamination.
This chapter discusses the purpose principle of operation specifications and applications of biosafety cabinet bsc.
Use of biological safety cabinets bsc conduct all procedures involving the manipulation of potentially infectious materials including aerosol producing activities when using aerosol producing equipment or when using high concentrations large volumes of organisms inside a biological safety cabinet.
The term containment describes safe methods or properly managing infectious agents in the laboratory environment where they are being handled or maintained.
Biological safety cabinet a piece of equipment designed to protect the operator the laboratory environment and work materials from exposure to infectious aerosols and splashes that may be generated when manipulating substances containing infectious agents such as viruses bacteria and primary tissue cultures.
Environmental protection from contaminants contained within the cabinet.
Product protection to avoid contamination of the work experiment or process.
Follow these guidelines for working safely in a biological safety cabinet bsc.
Improper use of a biological safety cabinet can result in contaminated cultures and expose workers to infectious organisms.
Personnel protection from harmful agents inside the cabinet.
When used correctly a curtain of room air enters the grill at the front edge of the bsc work surface and acts as a protective barrier.
A bsc serves as a means to protect the laboratory worker and the surrounding e.
In varying degrees a laminar flow biological safety cabinet is designed to provide three basic types of protection.
Primary containment which is the protection of personnel and the immediate laboratory environment from exposure to infectious agents is provided.
Improved principles ofbiological safety cabinet design true laminar airflow unidirectional airflow moving along parallel flow lines at a constant velocity minimizes air turbulence within the work zone.
The video describes the biosafety cabinet its principles operation uses maintenance calibration and safety methods.
